Method for the disruption of lipid-containing viruses
AssigneeAmerican Home Products Corporation
InventorAlexander R. Neurath
Life of this patent
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Abstract
Virus-containing media are treated and the viruses broken down into sub-units by mixing with trialkyl phosphates in the presence of a wetting agent. In comparison with conventional means, sub-unit vaccines prepared therefrom have low toxic pyrogen content, and influenza viruses of Types A and B are disrupted without substantial decreases in neuraminidase level. The method can be used on viruses contained in relatively dilute allantoic fluids as well as those in concentrated centrifugates.
Claims16
In a method for the disruption of infectious lipid-containing viruses for preparing sub-unit vaccines, the step of bringing the intact infectious lipid-containing virus in an aqueous medium into contact with effective amounts of a wetting agent and a trialkylphosphate wherein said alkyl groups contain from 4 to 10 carbon atoms.
In a method for the disruption of infectious lipid-containing viruses for preparing sub-unit vaccines, the steps of bringing the intact infectious lipid-containing virus in an aqueous medium into contact with effective amounts of a wetting agent and a trialkylphosphate wherein said alkyl groups contain from 4 to 10 carbon atoms, and separating the aqueous medium and disrupted virus from the said trialkylphosphate.
In a method for the disruption of influenza viruses in an allantoic fluid, for preparing sub-unit vaccines, the steps of bringing the intact virus in said fluid into contact with an effective amount of tri-(n-butyl)phosphate dispersed in said fluid with an effective amount of polyoxyethylene sorbitan mono-oleate, and separating the fluid and disrupted virus from the said trialkyl phosphate.
In a method for the disruption of infectious lipid-containing viruses for preparing sub-unit vaccines, the steps of bringing the intact infectious lipid-containing virus into contact on a calcium phosphate chromatographic column with an aqueous solution containing effective amounts of from about 0.05 to about 0.25% by weight of a wetting agent and from about 0.1% to about 1% by volume of a trialkylphosphate wherein said alkyl groups contain from 4 to 10 carbon atoms, and eluting with phosphate buffer having a pH of from about 6 to about 8.
Dependencies are inferred from the claim text.