Novel antibiotic and a process for the production thereof
AssigneeEli Lilly and Company
InventorsRobert L. Hamill, William M. Stark, Donald C. DeLong
Life of this patent
Estimating the term…
Abstract
Antibiotic A-4696, produced by Actinoplanes sp., strain ATCC 23342, under submerged aerobic conditions in a liquid culture medium, isolated from the fermentation broth by adsorption on activated carbon, eluted therefom with a 1% sulfuric acid solution in acetone, and purified over sulfuric acid-washed alumina has antibacterial and growth promotant activity.
Claims4
The antibiotic substance A-4696, or a pharmaceutically acceptable acid addition salt thereof, which antibiotic in the form of its hydrochloride salt is a white, crystalline substance soluble in water and insoluble in the common organic solvents, has a melting point greater than 220.degree. C.;
- has an electrometric titration curve in 66 percent aqueous dimethylformamide approximating a straight line with a slope of about 0.14 from pH 6.0 to pH 13.0;
- has the approximate composition of 51.33 percent carbon, 5.79 percent hydrogen, 5.46 percent nitrogen, 30.96 percent oxygen, and 6.72 percent chlorine;
- has an apparent molecular weight of 1158, as determined by the vapor pressure osmotic method;
- had a specific rotation, [.alpha]sub.D.sup.25 -42.3.degree. (c=1, H.sub.2 O);
- has an ultraviolet absorption spectrum in acidic and neutral solutions at 276 m.mu.. with an extinction coefficient E.sub.1cm. 1% of 65;
- and has the following distinguishable bands in its infrared absorption spectrum when determined in a mineral oil mull:
- 3.0, 5,8, 5.9, 6.03, 6.15, 6.28, 6.63, 6.85, 7.27, 7.75, 8.1, 8.25, 8.9, 9.4, 9.9, and 10.1 microns.
Dependencies are inferred from the claim text.