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US 3,948,732 AGranted
Cell culture assembly
AssigneeInstrumentation Laboratory Company
InventorsIhsan A. Haddad, Alvin R. Arsenault
Life of this patent
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AssigneeInstrumentation Laboratory Company
InventorsIhsan A. Haddad, Alvin R. Arsenault
Estimating the term…
A replaceable, sterilizable, cell growth assembly comprising a chamber structure of gas permeable, liquid impermeable material having an inner surface to which cells are attachable. The chamber is of tubing configuration and is disposed in a plurality of layers in stacked relation. Spacer structure between the layers of tubing define a plurality of flow passages between the layers and enable a gaseous environment to bathe the majority of the external surface area of the tubing layers. The chamber also includes an inlet conduit for introducing culture media for flow through the plural layers of the chamber structure and an outlet conduit for receiving culture media from the chamber structure, and coupling structure for detachably connecting the inlet conduit to a source of culture media and the outlet conduit to an appropriate culture media receptacle.
A replaceable, sterilizable cell growth assembly comprising a tubular chamber of gas permeable liquid impermeable material having an inner surface to which cells adhere, said chamber being disposed in a plurality of layers in stacked relation, spacer structure between adjacent layers of said chamber maintaining said adjacent layers spaced from one another to permit gas to contact external surfaces of said layers over substantially their entire lengths, supply conduit structure connected to said plural layer chamber for introducing culture media for flow through each plural layer chamber, and return conduit structure connected to said plural layer chamber for receiving culture nutrient media from said plural layer chamber structure, said supply and return conduits each being adapted to be detachably connected to cooperating structure.
A replaceable, sterilizable cell growth assembly comprising a tube of gas permeable, liquid impermeable organic plastic that has a roughed inner surface to which cells adhere, said tube being disposed in a plurality of layers in stacked relation, portions of said inner surface in each layer being disposed in opposed spaced relation and defining a passage for flow of culture media through said passage, spacer structure between adjacent layers of said tube maintaining the spacing of said opposed inner surface portions from one another to provide said culture media flow passage and the spacing of said adjacent layers from one another to permit gas to contact external surfaces of said tube over substantially the entire length of said tube, supply conduit structure connected to one end of said tube for introducing culture media for flow through said tube, and return conduit structure connected to the other end of said tube for receiving culture media from said tube, said supply and return conduits each being adapted to be detachably connected to cooperating structure for flowing culture media under pressure through said tube to nourish cells attached to said inner surface portions.
Dependencies are inferred from the claim text.