Preparation of anti-.alpha.1-fetoglobulin
AssigneeKowa Company, Ltd.
InventorsHidetaka Nagai, Eiichi Sato, Fujio Kobayashi
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Abstract
A diagnostic agent for primary hepatoma which has anti-.alpha.1-fetoglobulin, and a process for the preparation of the diagnostic agent for primary hepatoma which comprises the steps of dissolving the anti-.alpha.1-fetoglobulin together with a support medium and allowing the same to solidify in a diffused condition.
Claims4
A process for the preparation of anti-.alpha..sub.1 -fetoglobulin which is used for the preparation of a diagnostic agent for primary hepatoma which comprises:
- a. purifying the ascites from a patient having a primary hepatoma by means of gel filtration chromatography;
- b. dialyzing the .alpha..sub.1 -fetoglobulin fraction obtained from purifying step (a) so as to obtain .alpha..sub.1 -fetoglobulin;
- c. immunizing animals with said .alpha..sub.1 -fetoglobulin of step (b);
- d. bleeding said immunized animals;
- e. separating an anti-.alpha..sub.1 -fetoglobulin-containing serum from the blood of said bled animals;
- and
- f. absorbing said serum with normal human serum or insoluble normal human serum immunoabsorbent.
A process for the preparation of anti-.alpha..sub.1 -fetoglobulin which is used for the preparation of a diagnostic agent for primary hepatoma which comprises:
- a. purifying the ascites from a patient having a primary hepatoma by means of dialysizing an aqueous solution of said ascites in a ratio of 3-4:1 water to ascites against an aqueous solution of 0.1 - 0.01 W/V% acrinol;
- b. purifying the precipitate formed in step (a) by means of isoelectric fractionation or gel filtration chromatography to obtain the .alpha..sub.1 -fetoglobulin;
- c. immunizing animals with said .alpha..sub.1 -fetoglobulin of step (b);
- d. bleeding said immunized animals;
- e. separating an anti-.alpha..sub.1 -fetoglobulin-containing serum from the blood of said bled animals;
- and
- f. absorbing said serum with normal human serum or insoluble normal human serum immuno-absorbent.
Dependencies are inferred from the claim text.