Process for isolation of insulin from plant source
OwnerPushpa Khann, Tej Narain Nag +2inventors
InventorsPushpa Khann, Tej Narain Nag, Satish Chandrajain, Suchendra Mohan
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Abstract
A method for isolating insulin from the cultured seeds or fruit of Memordica charantia Linn in which the starting material is extracted with ethanol, water and sulfuric acid, adjusting the pH of the extract to 1.5 to 2.0 and then precipitating the insulin by adding cold ethanol and diethyl ether. Zinc traces are added to precipitate the insulin as white crude crystals which are then purified to white needle-like crystals. Comparison of this product with standard insulin by chromatography showed that the present product is substantially the same as the standard insulin.
Claims6
A process for isolating insulin from Memordica charantia Linn comprising extracting an organic material selected from the group consisting of cultures raised from seeds of Memordica charantia Linn and the fruit of Memordica charantia Linn with a mixture of ethanol, water and sulfuric acid, separating the extract from remaining material, adjusting the pH of said extract to 1.5 to 2.0 with ammonia or hydrochloric acid, adding cold ethanol and diethyl ether to precipitate insulin therefrom, adding zinc traces to the precipitate to crystallize the insulin as white crude crystals, and finally purifying the white crude crystals to white needle-like crystals.
Dependencies are inferred from the claim text.