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US 3,944,537 AGranted
Preparation of alpha-amylase inhibitor
AssigneeThe United States of America as represented by the Secretary of Agriculture
InventorsRobert M. Saunders, George O. Kohler
Life of this patent
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Abstract
Alpha-amylase inhibitor is prepared from whole wheat or other wheat material by blending the starting material with aqueous alkali, separating the juice therefrom, coagulating and separating a protein concentrate from the juice, and then applying acetone to the residual juice to precipitate the alpha-amylase inhibitor.
Claims2
A process for preparing alpha-amylase inhibitor, which comprises
- a. applying an aqueous alkalizer to wheat material to adjust to a pH 8 to 9,
- b. pressing a juice containing soluble proteins therefrom,
- c. acidifying the juice to a pH of 5.0 to 6.0,
- d. coagulating a solid protein concentrate within the juice by applying steam to the juice until its temperature is raised to 80.degree. C.,
- e. separating the so-coagulated solid protein concentrate from the juice,
- f. adding acetone to the residual juice to precipitate the alpha-amylase inhibitor contained therein, and
- g. separating the precipitated alpha-amylase inhibitor from the juice.
4 more elements
A process for preparing from wheat millfeed both alpha-amylase inhibitor and a stable protein concentrate useful as a human food supplement, which comprises
- a. applying an aqueous alkalizer to the wheat millfeed to adjust to a pH of 8 to 9,
- b. pressing a juice containing soluble proteins therefrom,
- c. acidifying the juice to a pH of 5.0 to 6.0,
- d. coagulating a solid protein concentrate within the juice by applying steam to the juice until its temperature is raised to 80.degree. C.,
- e. separating the so-coagulated solid protein concentrate from the juice,
- f. adding acetone to the residual juice, in the proportion of 2 volumes acetone per volume of juice, to precipitate the alpha-amylase inhibitor contained therein, and
- g. separating the precipitated alpha-amylase inhibitor from the juice.
4 more elements
Dependencies are inferred from the claim text.