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AssigneeKikkoman Shoyu Co., Ltd.
InventorsJiro Ishiyama, Tamotsu Yokotsuka, Motohiko Kato, Nobuyuki Yamaji, Fumihiko Yoshida
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3' ,5'-Cyclic-adenylic acid or 3' ,5'-cyclic-deoxadenylic acid can be obtained in a high purity and a high yield by adding a water-soluble organic solvent such as methyl alcohol, ethyl alcohol, n-propyl alcohol, isopropyl alcohol, tert-butyl alcohol, acetone, N,N'-dimethylformamide, dioxane, tetrahydrafuran, ethyleneglycolmonomethylether and the like singly or in combination to an aqueous solution containing as impurities one or more kinds of nucleotides other than 3' ,5'-cyclicadenylic acid and 3' ,5'-cyclic-deoxyadenylic acid, nucleosides, nucleobases, colored substances, proteins and the like in addition to 3' ,5'-cyclic-adenylic acid or 3', 5'-cyclic-deoxyadenylic acid. The said 3' ,5'-cyclic-adenylic acid or 3' ,5'-cyclic-deoxyadenylic acid are useful and valuable as a biochemical reagent.
A method for purifying 3', 5'-cyclicadenylic acid or 3', 5'-cyclic-deoxyadenylic acid comprising treating an aqueous solution containing 3', 5'-cyclic-adenylic acid or 3', 5'-cyclic-deoxyadenylic acid and impurities selected from the group consisting of nucleotides other than 3', 5'-cyclic-adenylic acid and 3', 5'-cyclic-deoxyadenylic acid, nucleosides, nucleobases, colored substances, proteins, and mixtures thereof by adjusting the pH to pH 5 or more, and adding a water-soluble organic solvent, said solvent being added in an amount sufficient to separate out or precipitate said impurities at said pH 5 or more while maintaining 3', 5'-cyclic-adenylic acid or 3', 5'-cyclic-deoxyadenylic acid in solution, and removing the separated or precipitated impurities from said solution.
A method for purifying 3', 5'-cyclic-adenylic acid or 3', 5'-cyclic-deoxyadenylic acid comprising treating an aqueous solution containing 3', 5'-cyclic-adenylic acid or 3', 5'-cyclic-deoxyadenylic acid and impurities selected from the group consisting of nucleotides other than 3', 5'-cyclic-adenylic acid and 3', 5'-cyclicdeoxyadenylic acid, nucleosides, nucleobases, colored substances, proteins and mixtures thereof by adjusting the pH of said solution to pH 5 or more, and adding a water-soluble organic solvent in an amount sufficient to separate out or precipitate said impurities at said pH 5 or more while maintaining 3', 5'-cyclic-adenylic acid or 3', 5'-cyclic-deoxyadenylic acid in solution, removing the separated or precipitated impurities from said solution and thereafter recovering 3', 5'-cyclic-adenylic acid or 3', 5'-cyclic-deoxyadenylic acid from said solution.
A method for purifying 3', 5'-cyclic-adenylic acid or 3', 5'-cyclic-deoxyadenylic acid consisting essentially of adjusting an aqueous solution containing 3', 5'-cyclic-adenylic acid or 3', 5'-cyclicdeoxyadenylic acid, at least one impurity selected from the group consisting of proteins and nucleotides other than 3', 5'-cyclic-adenylic acid and 3', 5'-cyclic-deoxyadenylic acid and at least one impurity selected from the group consisting of nucleosides, nucleobases and colored substances to pH 5-12, adding at least one water-soluble organic solvent selected from the group consisting of methyl alcohol, ethyl alcohol, n-propyl alcohol, isopropyl alcohol, tert.-butyl alcohol, acetone, n, n'-dimethylformamide, dioxane, tetrahydrofuran and ethyleneglycolmonomethylether in an amount sufficient to separate or precipitate said impurities with maintaining said 3', 5'-cyclic-adenylic acid or 3', 5'-cyclic-deoxyadenylic acid in solution, separating the formed separated materials or precipitates therefrom, adjusting the pH of the aqueous solution to 3 or less and then collecting the crystallized 3', 5'-cyclic-adenylic acid or 3', 5'-cyclic-deoxyadenylic acid from the solution.
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