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AssigneeGist-Brocades N.V.
InventorsSophia Adriana Lameris, Jan Lambert VAN Os, Joannes Gerard Oostendorp
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A method for the rapid determination of the presence or absence of residues of antibiotic such as penicillin, in liquids, for example milk, and meat comprising introducing spores of a microorganism which possesses a high sensitivity for the antibiotic to be determined into an agar medium so that the spores are prevented from starting to germ due to lack of nutrients, but stay alive, and being sufficiently heavily inoculated that after addition of nutrients and incubation at or near optimal temperature in a short time sufficient growth has proceeded so that growth is either observable visually or its indicated by an indicator added to the agar medium, the said spore culture being allowed to solidify in upright test vessels having a cross-sectional dimension sufficiently small to require as little of the agar medium as possible but sufficiently large to enable a correct determination of inhibition of growth of the microorganism to be made, and having a height so that the vessel can contain a sufficient amount of medium and a sufficient amount of sample of liquid to be tested, placing on the agar surface nutrients required for growth, followed, if desired, after a pre-incubation period, by addition of a predetermined amount of sample liquid to be tested to the test vessel, and incubating the contents of the test vessel at or near optimal temperature for a predetermined time so that the extent of growth or inhibition of growth of the microorganism read in a vertical sense, indicates absence or presence of the antibiotic.
A method for the rapid determination of the presence or absence of residues of antibiotic in liquids and meat comprising introducing spores of a microorganism which possesses a high sensitivity for the antibiotic to be determined in an agar medium so that the spores are prevented from starting to germ by lack of nutrients, but stay alive, and being sufficiently heavily inoculated that after addition of nutrients and incubation at or near optimal temperature in a short time sufficient growth has proceeded that growth is indicated by an indicator added either to the agar medium or to the nutrients, the resulting spore and agar mixture being allowed to solidify in upright test vessels having a cross-sectional dimension sufficiently small to require as little of the agar medium as possible, but sufficiently large to enable a correct determination of inhibition of growth of the microorganism to be made and having a height so that the vessel can contain a sufficient amount of medium and a sufficient amount of sample liquid to be tested, placing on the agar surface dried nutrients, in the form of a filter paper disc or tablet, required for growth, adding a predetermined amount of sample liquid to be tested to the test vessel, and incubating at or near optimal temperature the contents of the test vessel for a predetermined time so that the extent of growth or inhibition of growth of the microorganism read in a vertical sense indicates absence or presence of antibiotic.
Dependencies are inferred from the claim text.