Purification of d-aminoacid oxidase
AssigneeRhone-Poulenc Industries
InventorsHonore Mazarguil, Francois Meiller, Pierre Monsan
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Abstract
Process for the separation and purification of d-aminoacid oxidase by affinity chromatography. The process comprises contacting a mixture of proteins containing the d-aminoacid oxidase with a mineral carrier carrying haloalkylsilane grafts, upon which are fixed -- NH -- (CH.sub.2).sub.3 -- NH -- (CH.sub.2).sub.2 C.sub.6 H.sub.4 COOH residues. The enzyme retained by the carrier is then separated by a solution of ethyleneglycol. Use of the pure d-aminoacid oxidase for the preparation of cetonic acids.
Claims4
A process for the separation and purification of d-aminoacid oxidase, which comprises contacting a solution of a mixture of proteins containing the d-aminoacid oxidase with a carrier onto which the enzyme is fixed, then separating the d-aminoacid oxidase from the carrier, characterized in that the carrier is a mineral carrier bearing haloalkylsilane grafts modified by fixing residues having the formula --NH -- (CH.sub.2).sub.3 -- NH -- (CH.sub.2).sub.2 C.sub.6 H.sub.4 COOH, and that the purified d-aminoacid oxidase is separated from the carrier by dissolution in a solution of ethyleneglycol.
Dependencies are inferred from the claim text.