Novel antibiotic BN-109 substance, its production and use

An antibiotic BN-109 substance, which is effective in inhibiting the growth of Gram-negative bacteria and having the following properties:

• Appearance:
• White powder as a free base;
• Solubility:
• Insoluble in common organic solvents and water, slightly soluble in diluted acid and alkaline, acetic acid dimethylformamide and dimethyl sulfoxide, and soluble in acidic methanol and acidic ethanol;
• Mobility on filter paper electrophoresis:
• Behaves as an alkaline substance;
• Rf values on TLC:
• 0.86 (n-butanol-acetic acid-water (2:1:1)),
• 0.38 (n-butanol-acetic acid-water (4:1:5, upper layer)),
• 0.66 (n-butanol-pyridine-acetic acid-water (6:4:1:3));
• [.alpha]sub.D.sup.25 (c 0.5, methanol-acetic acid (1:1)):
• -4.degree.;
• Maximum UV absorption bands:
• Found at 279 m.mu. (E.sub.1cm.sup.1% = 34, in 0.1 N-NaOH, E.sub.1cm.sup.1% = 23 in 0.1 N-HCl);
• Ir absorption bands:
• Found at 3275, 2590, 1632, 1535, 1408, 1110 (cm.sup..sup.-1).
• Color reactions:
• Positive ninhydrin, Ehrich and Dragendorff;
• Negative Sakaguchi, Pauly and Trevelian;
• Elementary analysis:
• C, 52.73%;
• H, 7.27%;
• N, 12.77%;
• Amino acid composition:
• .alpha.,.gamma.-diamino butyric acid(4), serine(2), glutamic acid(1), glycine(1), alanine(1), valine(3), phenylalanine(1), tryptophan(2), and unknown animo acid(1);
• Fatty acids:
• Contain 30% of acid ether-soluble fatty acids;
• Molecular weight:
• 1900 to 2200