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US 3,940,317 AGranted
Method of isolation of lysozyme
OwnerFriedrich D. Katz, Louis Fishman +1inventors
InventorsFriedrich D. Katz, Louis Fishman, Milton Levy
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Abstract
Lysozyme from human, avian, non-human mammalian, and vegatable sources is isolated from said sources and provided in a high degree of purity by adsorbing the lysozyme upon chitin obtained from Loligo vulgaris by contacting said chitin with an acidic aqueous suspension of the crude lysozyme containing material, and eluting the pure lysozyme from said chitin with aqueous acid.
Claims19
A method of isolating lysozyme from a source thereof comprising:
- a. treating an aqueous suspension of said lysozyme containing source material with deaminated squid chitin,
- b. separating the chitin from said suspension, washing said chitin with aqueous acid and separating said aqueous acid from said chitin.
Dependencies are inferred from the claim text.