Testing using luminescent reaction
AssigneeVitatect Corporation
InventorsChris J. Plakas, George S. Glaros
Life of this patent
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Abstract
A method and apparatus for testing a sample by means of luminescent reaction to detect reactive materials therein. A light-transparent filter is provided to filter the sample and to carry the residue to a reaction position. A light-transparent surface is provided to carry a reagent to the reaction position. Both surfaces are brought into mutual contact in a region between two photodetectors, which thereby detect the resulting luminescent reaction to provide an indication of the quantity of reactive material present in the sample.
Claims13
A method of optically detecting the presence of biological cells as reactive materials in a sample containing a plurality of biological cells comprising the steps of:
- A. filtrating said sample on a light-transmitting filter to produce a filtrate containing biological cells from the sample,
- B. rupturing the cells in the residue while on said filter,
- C. depositing a reagent on a light-transmitting surface,
- D. bringing said surface and said filter into contact for luminescent reaction, and
- E. detecting said luminescence as an indication of said biological cells as reactive materials present in said sample.
A surface reaction system for detecting reactive materials in a test sample containing a plurality of individual biological cells by means of luminescent reaction, comprising:
- A. means for filtering said sample through a filter to produce a residue containing biological cells from the sample,
- B. extraction means for rupturing the cells in the residue while on said filter,
- C. means for bringing reagent material into contact with the ruptured cells on the filter to cause reaction and consequent bioluminescence, and
- D. detector means for measuring and indicating the intensity of the bioluminescence, thereby indicating the quantity of biological cells in the sample.
A surface reaction system for detecting biological cells as reactive materials in a test sample containing a plurality of biological cells by means of luminescent reaction, comprising:
- A. means for filtrating said sample through a light-transmitting filter to produce a residue containing biological cells from the sample,
- B. extraction means for rupturing the cells in the residue while on said filter,
- C. means for depositing reagent material on a light-transmitting surface,
- D. means for bringing said light-transmitting filter with the ruptured cells of said residue thereon into contact with said light transmitting surface with said reagent material thereon, thereby allowing reaction and consequent luminescence, and
- E. detector means for recording the intensity of said luminescence, thereby indicating the quantity of biological cells as said reactive materials present in the sample.
Dependencies are inferred from the claim text.