Back to results
US 3,935,074 AGranted
Antibody steric hindrance immunoassay with two antibodies
AssigneeSyva Company
InventorsKenneth Edward Rubenstein, Richard K. Leute
Life of this patent
Estimating the term…
Loading patent…
Loading patent…
AssigneeSyva Company
InventorsKenneth Edward Rubenstein, Richard K. Leute
Estimating the term…
A novel immunoassay is provided, as well as particular reagents, for determining the presence of a ligand. A Reagent is employed having at least two epitopes, one of the epitopes being common with the ligand, and the other epitopes being foreign to the ligand. The two epitopes are positioned in the Reagent so that antibody bound to one of the epitopes sterically inhibits the binding of antibody to the second epitope. In carrying out the assay, the sample, the Reagent, and antibodies to the two epitopes are combined. Because of the steric inhibition to the simultaneous binding of the two antibodies to the Reagent, the amount of antibody bound to the epitope of the Reagent foreign to the ligand will be related to the amount of ligand present in the assay medium. By analyzing directly or indirectly for the antibody bound to the epitope foreign to the ligand, and comparing the results to known standards, qualitative or quantitative determinations of the amount of ligand may be made. Various detector systems may be employed for determining the amount of antibody to the foreign epitope which is unbound or bound. These systems include stable free radicals, enzymes, radioactive labels, fluorescers, and the like.
A method for determining the presence of a ligand in an unknown suspected of containing said ligand which comprises:
A method for determining the presence of a ligand in an unknown suspected of containing said ligand which comprises:
A method for determining the presence of a ligand in an unknown suspected of containing said ligand which comprises:
Dependencies are inferred from the claim text.