Cell lines
AssigneeBurroughs Wellcome Co.
InventorKostadin Apostolov
Life of this patent
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Abstract
A human epithelial heterploid liver cell line, such as line WRL 68 which forms individually separated islands or discrete clumps when cultured in a growth medium, has a morphology closely resembling that of hepatocytes of the human liver and a generation time not more than 24 hours, manifests increased production of glycogen in the presence of 1% glucose in the medium, and is capable of supporting viruses. Such cells may be advantageously used to provide cell cultures for supporting viruses for the preparation of viral vaccines.
Claims4
A cell culture of a human epithelial heteroploid liver cell line, comprising a cell line in association with a nutrient culture medium, said cell line being a human epithelial heteroploid liver cell line, comprising cells characterized as follows:
- a. the cells form individually separated islands or discrete clumps when cultured in a growth medium;
- b. the cells have a morphology closely resembling that of hepatocytes of the human liver;
- c. the cells have a generation time not more than 24 hours;
- d. the cells manifest increased production of glycogen in the presence of 1% glucose in the said growth medium;
- and
- e. the cells are capable of supporting viruses.
A cell culture of a human epithelial heteroploid liver cell line, comprising a cell line in association with a nutrient culture medium, said cell line comprising a human epithelial heteroploid liver cell line, as deposited with the American Type Culture Collection under accession number CL48.
Dependencies are inferred from the claim text.