Solid phase radioimmunoassay of digoxin
AssigneeCorning Glass Works
InventorsLeroy S. Hersh, Sidney Yaverbaum
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Abstract
Clinically significant concentrations of digoxin can be determined by reacting an unknown amount of digoxin, a known amount of labelled digoxin, and a composite consisting of anti-digoxin antibodies coupled through an intermediate silane to magnetically responsive particles; magnetically separating the immunochemical complexes formed thereby, counting the radioactivity of either the separated products or remaining solution, and relating the count to a standard curve.
Claims7
A method of determining the concentration of digoxin in a solution comprising the steps of:
- A. reacting the solution, a known amount of radioactively labelled digoxin, and a composite consisting of anti-digoxin antibodies coupled chemically through an intermediate silane coupling agent to magnetically responsive inorganic particles, to form immunochemical complexes;
- B. magnetically separating the composite from the reaction solution;
- C. counting the radioactivity of either the removed composite or the remaining solution;
- and
- D. relating the count of step (C) to a standard to determine the digoxin concentration.
An immunochemical composite suitable for use in determining clinically significant concentrations of digoxin and comprising anti-digoxin antibodies chemically coupled through an intermediate silane coupling agent to particles of Fe.sub.3 O.sub.4 having an average particle size between about 1.5 and about 10 microns.
Dependencies are inferred from the claim text.