Display Substrate and Manufacturing Method Therefor, and Display Device

A display substrate, comprising a drive structure layer disposed on a base substrate and a light emitting structure layer disposed on a side of the drive structure layer away from the base substrate, wherein on a plane parallel to the display substrate, the drive structure layer comprises a plurality of circuit units, at least one circuit unit comprises a pixel drive circuit, a power supply connection line extending along a first direction, and a first power supply line extending along a second direction, the first direction and the second direction are intersected, the pixel drive circuit comprises at least a power supply connection electrode connected to the power supply connection line, the first power supply line is connected to the power supply connection line or the power supply connection electrode through a power supply via;

• the light emitting structure layer comprises a plurality of light emitting units, at least one light emitting unit comprises an anode and a pixel definition layer disposed on a side of the anode away from the base substrate, the anode is connected with a pixel drive circuit of a corresponding circuit unit, the pixel definition layer is provided with a pixel opening exposing the anode, and an orthographic projection of at least one pixel opening on the base substrate is not overlapped with an orthographic projection of the power supply via on the base substrate.