Display substrate, manufacturing method and display device

A display substrate, comprising:

• a base substrate;
• and
• a pixel definition layer and a plurality of light-emitting subpixels located on the base substrate, wherein the pixel definition layer defines a plurality of pixel aperture regions and a plurality of communication regions, the light-emitting subpixel in the plurality of light-emitting subpixels is located in a corresponding pixel aperture region in the plurality of pixel aperture regions, at least part of pixel aperture regions adjacent to each other are in communication with each other via a corresponding communication region in the plurality of communication regions, and the communication region is configured to serve as a channel through which printing ink for the light-emitting subpixel passes;
• wherein
• the communication region comprises a first side and a second side arranged opposite to each other in an extension direction of the channel, the channel of the communication region comprises a first channel region located at the first side, a second channel region located at the second side, and a middle channel region located between the first channel region and the second channel region, a width direction of an orthogonal projection of the communication region onto the base substrate is perpendicular to the extension direction of the channel, and a width of at least one of the first channel region and the second channel region is greater than a width of the middle channel region.