Methods and tools for detecting the impact of compounds on protein, rna or dna interactions

An in vitro method for evaluating the ability of a compound to disrupt or stabilize an interaction between one or more bait and one or more candidate prey in a eukaryotic cell, comprising the steps of:

• a. providing a eukaryotic cell expressing (i) one or more bait, and (ii) one or more candidate prey, wherein said bait comprises a bait moiety and a polymerized tubulin-binding moiety;
• b. determining the occurrence of an interaction between said one or more bait and said one or more candidate prey in the eukaryotic cell, wherein said bait is bound to polymerized tubulin in the eukaryotic cell, thereby localizing said one or more candidate prey along said polymerized tubulin, thereby detecting said interaction;
• c. contacting the said eukaryotic cell with the said compound;
• d. determining the occurrence of the interaction between said one or more bait and said one or more candidate prey in the eukaryotic cell as in step b. and quantify this interaction;
• e. Comparison of the occurrences of the interaction made in step b. and in. step d.;
• f. Conclude whether the effect of the said compound is the disruption or the stabilization of the interaction between said one or more bait and one or more candidate prey.