Sixteen color natural killer cell immunoprofiling panel and reagent kit for spectral flow cytometers

Abstract

In one embodiment, a method of building an optimized color flow cytometry panel is disclosed using a spectral flow cytometer with three excitation lasers and thirty-eight color detectors. In another embodiment, a graphical user interface is disclosed generated by a server computer from a fluorochrome database and displayed by a client computer to assist in the selection of a set of fluorochromes for use in an assay to analyze biological samples. The GUI can display spectra graphs to visually show how fluorochromes may overlap and can generate similarity indexes for the paired fluorochrome interference and a complexity index for overall many to many interferences generated by a selected group or set of fluorochromes.

Dependencies are inferred from the claim text.