Molecular encryption of dna-encoded data in microcompartments
AssigneeTechnische Universiteit Eindhoven
InventorsBastiaan Wilhelmus Albertus BÖGELS, Tom Franciscus Antonius DE GREEF
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Abstract
Highly advantageous means and methods are provided to protect data, i.e. through physical molecular encryption of nucleic-acid encoded data. Briefly, digital data, i.e. information is first encoded and designed into nucleic acid-encoded data; based on this nucleic acid-encoded data polynucleotide fragments are made. The nucleic acid encoded data is molecularly encrypted, i.e. molecularly locked. Molecularly locked means that access to the information in the nucleic acid encoded data, once converted into nucleic acid molecules, is blocked, which lockage can be unlocked by providing a specific unlocker, a molecule, that functions as a key to gain access to the nucleic acid.
Claims22
Physically molecularly encrypted nucleic acid-encoded data comprising:
- a) a plurality of double stranded polynucleotide fragments, comprising primer binding sites, said primer binding sites flanking nucleic acid encoded data
- b) wherein at least a fraction of the double stranded polynucleotide fragments comprises one or more locker target sequences, optionally wherein the locker target sequence(s) and/or the primer binding site(s) of the polynucleotide sequence is/are part of the nucleic acid-encoded dataset
- c) a locker, which is an oligonucleotide, having a modification that blocks amplification, optionally wherein the modification of the locker that blocks amplification is selected from a 3′ inverted dT nucleotide a 3′ dideoxycytidine (ddC) a C3 phosphoramidite spacer, a 3′ amino modifier and a 3′ phosphorylation, preferably a 3′ inverted dT nucleotide;
- d) wherein the locker has sequence complementarity to the locker target in the double stranded polynucleotide fragments, and binds thereto, thereby preventing PCR-amplification of said double stranded polynucleotide fragments with primers from the primer binding sites;
- e) wherein the plurality of double stranded polynucleotide fragments and the locker are mixed.
A method for physical molecular encryption of nucleic acid-encoded data, the method comprising the steps:
- a) providing a digital dataset;
- b) encoding the digital dataset to a nucleic acid code, thereby designing a nucleic acid-encoded dataset, wherein the nucleic acid-encoded dataset comprises a plurality of polynucleotide sequences of between 50-500 nucleotides, preferably 100-250 nucleotides, more preferably 150-200 nucleotides, wherein each of the polynucleotide sequences further comprise a forward and reverse primer binding site at the ends of the polynucleotide sequences that allows for subsequent PCR amplification wherein up to 100% of the individual polynucleotide sequences comprise at least one locker target sequence wherein the locker target sequence is located such that PCR amplification is blocked when a locker is bound to the locker target sequence, optionally wherein the locker target sequence and/or the primer binding site(s) of the polynucleotide sequence is/are part of the nucleic acid-encoded dataset, and/or optionally wherein the polynucleotide sequences comprise an indexing sequence;
- c) synthesizing the designed plurality of polynucleotide sequences to provide for polynucleotide fragments, wherein the polynucleotide fragments are synthesized as single stranded polynucleotide fragments and subsequently made double stranded polynucleotide fragments or synthesized as double stranded polynucleotide fragments, preferably comprising an amplification step to provide for a desired concentration of double stranded polynucleotide fragments;
- d) labeling the double stranded polynucleotide fragments with biotin to therewith provide for biotin-labeled double stranded polynucleotide fragments representing the nucleic acid-encoded dataset.
-17. (canceled)
-27. (canceled)
Dependencies are inferred from the claim text.