Novel biosynthesis system for producing 3'-phosphoadenosine-5'-phosphosulfate and application thereof

Abstract

The present disclosure discloses a novel biosynthesis system for producing 3′-phosphoadenosine-5′-phosphosulfate and an application thereof, and belongs to the technical field of bioengineering. The present disclosure constructs an in vitro six-enzyme cascade reaction using ribose and adenosine as substrates by recombinantly expressing and purifying RBKS, PRS, APRT, APSST, APSK, and PPK2, achieves a molar conversion rate of 22.85%, and constructs PAPS-producing cell factories of Escherichia coli and Bacillus subtilis, with PAPS yields reaching 7.60 g/L and 5.03 g/L respectively through 5-L fermenter fermentation. The PAPS synthesis system provided by the present disclosure offers a scalable and cost-effective solution for production of sulfated compounds.

Dependencies are inferred from the claim text.