Method for producing d-talitol, d-tagatose, and d-psicose
AssigneeAnnikki GmbH
InventorsNicole STAUNIG, Maria DUPONT, Melanie TROBE
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Abstract
The invention consists of a method for the production of an aqueous solution containing D-talitol in that D-psicose is formed in vitro from D-fructose, which exists in a dissolved state in an aqueous solution, by treating it with an epimerase, whereupon the D-psicose is reduced in vitro to D-talitol by treating it with an NAD(P)H-dependent oxidoreductase. The D-talitol contained in the aqueous solution can easily be oxidized to D-tagatose or D-psicose by treating it with an oxidoreductase.
Claims23
A method for the production of an aqueous solution containing D-talitol in that D-psicose is formed in vitro from D-fructose, which exists in a dissolved state in an aqueous solution, by treating it with an epimerase, whereupon the D-psicose is reduced in vitro to D-talitol by treating it with an NAD(P)H-dependent oxidoreductase, and the epimerase and the oxidoreductase are separated or deactivated.
A method for the production of D-talitol or D-psicose, comprising the step of treating D-psicose or D-talitol with an oxidoreductase comprising an amino acid sequence selected from the group consisting of:
- i) an amino acid sequence having an identity to SEQ ID No. 2, SEQ ID No. 6, SEQ ID No. 8, SEQ ID No. 10, SEQ ID No. 12, SEQ ID No. 14, SEQ ID No. 16, SEQ ID No. 18, or SEQ ID No. 20 of at least 80%,
- ii) an amino acid sequence encoded by a nucleic acid having an identity to SEQ ID No. 1, SEQ ID No. 5, SEQ ID No. 7, SEQ ID No. 9, SEQ ID No. 11, SEQ ID No. 13, SEQ ID No. 15, SEQ ID No. 17, or SEQ ID No. 19 of at least 80%, and
- iii) an amino acid sequence encoded by a nucleic acid that binds under stringent conditions to a nucleic acid molecule having the nucleic acid sequence SEQ ID No. 1, SEQ ID No. 5, SEQ ID No. 7, SEQ ID No. 9, SEQ ID No. 11, SEQ ID No. 13, SEQ ID No. 15, SEQ ID No. 17, or SEQ ID No. 19.
A method for the production of D-talitol, comprising the step of treating D-psicose with an oxidoreductase comprising an amino acid sequence selected from the group consisting of:
- i) an amino acid sequence having an identity to SEQ ID No. 22 or SEQ ID No. 24 of at least 80%,
- ii) an amino acid sequence encoded by a nucleic acid having an identity to SEQ ID No. 21 or SEQ ID No. 23 of at least 80%, and
- iii) an amino acid sequence encoded by a nucleic acid that binds under stringent conditions to a nucleic acid molecule having the nucleic acid sequence SEQ ID No. 21 or SEQ ID No. 23.
(canceled)
(canceled)
A method for the production of D-tagatose, comprising the step of treating D-talitol with an oxidoreductase comprising an amino acid sequence selected from the group consisting of:
- i) an amino acid sequence having an identity to SEQ ID No. 4 of at least 80%,
- ii) an amino acid sequence encoded by a nucleic acid having an identity to SEQ ID No. 3 of at least 80%, and
- iii) an amino acid sequence encoded by a nucleic acid that binds under stringent conditions to a nucleic acid molecule having the nucleic acid sequence SEQ ID No. 3.
(canceled)
Dependencies are inferred from the claim text.