Click chemistry assisted cell surface engineering for restoring lentivirus entry
AssigneesThe Children's Hospital of Philadelphia, The Trustees of the University of Pennsylvania
InventorsLeyuan MA, Letitia CHAN
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Abstract
The present disclosure is directed to methods of labeling the surface of cells with a ligand for improved transduction. The cells may be labeled with a recombinant LDLR or anti-VSV-G antibody, such as by click chemistry. Further provided herein are methods of gene therapy, screening, and disease models using the present surface labeled cells.
Claims16
A method of chemically labeling a cell comprising attaching to the surface of said cell a recombinant low density lipoprotein receptor (LDLR) protein or anti-vesicular stomatitis virus envelope glycoprotein (VSV-G) antibody or fragment thereof by performing click chemistry.
A chemically labeled cell having a recombinant LDLR or anti-VSV-G antibody or fragment thereof attached to the surface.
(canceled)
Dependencies are inferred from the claim text.