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US 2026/0308601 A1Published application
Methods of increasing sialic acid levels in recombinant glycosylated proteins
AssigneeDenali Therapeutics Inc.
InventorsMelina LENSER, Hanh Giai NGO, Anh Thuy NGUYEN DANG, Allain Erfe PETERSEN, Yashas RAJENDRA
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Abstract
Provided herein are methods of increasing the sialic acid level in a recombinant glycosylated protein, recombinant glycosylated proteins produced by these methods, and methods of treating a subject in need thereof using these recombinant glycosylated proteins.
Claims37
A method of increasing the sialic acid level in a recombinant glycosylated protein, comprising:
- culturing a mammalian host cell capable of expressing the recombinant glycosylated protein in a liquid culture medium comprising one or more media additives selected from the group consisting of:
- hydrocortisone, N-acetylmannosamine (ManNAc), manganese, and uridine,
- wherein the mammalian host cell has been previously transformed with one or more vectors encoding one or more sialyltransferases.
Dependencies are inferred from the claim text.