Methods of engineering cells with mutated genomes using intact large cargo transfer systems

Abstract

Systems and methods method of cellular engineering are provided. The systems and methods can include transferring exogenous intact mitochondria into recipient cells. In some aspects, the systems and methods can include removing or depleting endogenous mitochondria from one or more recipient cells, mutating mtDNA within one or more donor cells, extracting mitochondria having mutated mtDNA from the one or more donor cells, transferring the mitochondria having mutated mtDNA into the one or more recipient cells, wherein the mitochondria are transferred intact, identifying one or more phenotypic changes of the one or more recipient cells, wherein the one or more phenotypic changes is associated with the transferred mitochondria, and isolating one or more of the recipient cells based on the identified phenotypic changes.

Dependencies are inferred from the claim text.