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US 2026/0308558 A1Published application
Xylanase variants and polynucleotides encoding same
AssigneeNovozymes A/S
InventorsAnnette Helle JOHANSEN, Steffen DANIELSEN, Frank Winther RASMUSSEN, Peter Kamp HANSEN, Roland PACHE
Life of this patent
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Abstract
Xylanase variants; polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; compositions comprising the xylanase variants and methods of using the variants.
Claims20
A process for producing a fermentation product, comprising:
- (a) saccharifying a starch-containing material at a temperature below the initial gelatinization temperature of said starch-containing material with an alpha-amylase, a glucoamylase, and a xylanase variant having xylanase activity and improved thermostability compared to a parent xylanase, wherein the variant has at least 85% sequence identity to SEQ ID NO:
- 1 and comprises one or more substitutions relative to the parent xylanase, said one or more substitutions occurring at one or more positions corresponding to positions 14, 18, 50, 145, 146, 176, 179, 197, 298 and 299 of SEQ ID NO:
- 1;
- and
- (b) fermenting the saccharified material using a fermenting organism.
2 more elements
A process for producing a fermentation product, comprising:
- (a) liquefying a starch-containing material with an alpha-amylase;
- (b) saccharifying the liquefied material obtained in step (a) with a glucoamylase and a xylanase variant having xylanase activity and improved thermostability compared to a parent xylanase, wherein the variant has at least 85% sequence identity to SEQ ID NO:
- 1 and comprises one or more substitutions relative to the parent xylanase, said one or more substitutions occurring at one or more positions corresponding to positions 14, 18, 50, 145, 146, 176, 179, 197, 298 and 299 of SEQ ID NO:
- 1;
- and
- (c) fermenting using a fermenting organism.
3 more elements
Dependencies are inferred from the claim text.