Novel pet-degrading enzyme and use thereof

A modified polypeptide having a polyethylene terephthalate (PET) degradation activity,

• wherein i) the modified polypeptide has at least 70% and less than 100% sequence identity to SEQ ID NO:
• 1;
• and/or
• ii) the modified polypeptide is a polypeptide encoded by a polynucleotide having at least 70% and less than 100% sequence identity to a coding sequence of a mature polypeptide of SEQ ID NO:
• 1;
• and/or
• iii) the modified polypeptide is a polypeptide encoded by a polynucleotide that hybridizes with (a) the coding sequence of the mature polypeptide of SEQ ID NO:
• 1, (b) cDNA thereof, or (c) the full-length complement of (a) or (b) under low stringency conditions, medium stringency conditions, medium-high stringency conditions, high stringency conditions, or very high stringency conditions;
• and/or
• iv) the modified polypeptide is a functional fragment of the polypeptide of i), ii), or iii) that has the PET degradation activity;
• and
• the modified polypeptide comprising any one modification selected from the following modifications:
• deletion of amino acids, insertion of the amino acids, substitution with different amino acids, formation of disulfide bonds, and/or a combination thereof at any one or more positions of positions 180, 202, 242, and 291;
• wherein the position number is a position corresponding to the position of the polypeptide of SEQ ID NO:
• 1.