Polymerase variants
AssigneeTwist Bioscience Corporation
InventorsOwen Kabnick SMITH, Sean Patrick TIGHE, Xuan Yu Elian LEE, Ramsey Ibrahim ZEITOUN, Siyuan CHEN
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Abstract
Described herein are methods and compositions relating to enzyme polypeptides and libraries having nucleic acids encoding for the polypeptides comprising variant amino acid sequences. Further described herein are methods of extending polynucleotide molecules using enzyme polypeptides having variant amino acid sequences. Further described herein are methods for preparing sequencing libraries using polymerase polypeptides having variant amino acid sequences.
Claims20
A polypeptide comprising an amino acid sequence comprising at least one amino acid mutation relative to SEQ ID NO:
- 1.
The polypeptide of any one of the preceding claims, wherein the polypeptide comprises at least 2, at least 3, or at least 4 amino acid mutations relative to SEQ ID NO:
- 1.
A method for extending a first polynucleotide, the method comprising:
- contacting the first polynucleotide with a nucleotide and a polypeptide to form an extended polynucleotide,
- wherein the polypeptide comprises an amino acid sequence comprising at least one amino acid mutation relative to SEQ ID NO:
- 1.
A method for preparing a sequencing library comprising:
- providing a plurality of nucleic acids;
- end-repairing the plurality of nucleic acids;
- performing a-tailing on the plurality of nucleic acids using a polymerase, wherein the polymerase comprises an amino acid sequence comprising at least one amino acid mutation relative to SEQ ID NO:
- 1;
- and
- ligating at least one adapter to the nucleic acids using a ligase.
Dependencies are inferred from the claim text.