Compositions and methods for propagating insulin- producing islet cells and therapeutic uses thereof cross reference to related applications
AssigneeImagine Pharma LLC
InventorsNGOC THAI, JONATHAN POLLETT
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Abstract
Disclosed herein are compositions and methods to differentiate pancreatic cells into functional insulin-producing CD133/Ki-67-positive activated islet proliferating cells (AIPCs) derived from isolated pancreatic islets and expand derived-AIPCs in in vitro cultures using a culture medium comprising an active agent. Also disclosed herein is the use of the AIPCs for implantation into a mammal for in vivo therapy, specifically for pancreatic disorders, including diabetes type I.
Claims9
A composition comprising an activated islet proliferating cell (AIPC) population, wherein 60% of the AIPC population is comprised of a CD133 cell marker and a Ki-67 cell marker, and
- wherein at least 60% of the AIPC population is capable of insulin production.
A composition comprising an activated islet proliferating cell (AIPC) population obtained by a process comprising:
- culturing an isolated pancreatic cell population in vitro with a cell culture medium comprising a base medium and an effective amount of a polypeptide according to SEQ ID NO 1 or 2 to generate the AIPC population,
- wherein at least 30% of the AIPC population is comprised of a CD133 cell marker and a Ki-67 cell marker.
Dependencies are inferred from the claim text.