Compositions and methods for propogating insulin and glucagon secreting cells from type 1 diabetic pancreatic tissue and therapeutic uses thereof

A method for preparing a composition comprising an insulin and glucagon secreting cell population, which comprises:

• treating in vitro a population of non-insulin secreting Type 1 diabetic pancreatic cells with an islet cell culture medium comprising a base medium and an effective amount of a polypeptide comprising an amino acid sequence having at least 80% sequence identity to the amino acid sequence set forth in SEQ ID NO. 1 or 2, a polypeptide comprising SEQ ID NO. 1, an active fragment of SEQ ID NO. 1, SEQ ID NO. 2, an active fragment of SEQ ID NO. 2, or a combination thereof.

A composition comprising an insulin and glucagon secreting cell population generated from non-insulin secreting pancreatic cells collected from a Type 1 diabetic donor pancreas for use in the treatment of Type 1 diabetes, pancreatitis, or a combination thereof.