Protocol for maturation of 3D engineered heart tissue
OwnerBhairab N. Singh, Mohammad Shameem +1inventors
InventorsBhairab N. Singh, Mohammad Shameem, Ahmed M. Sharara
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Abstract
A method and system for promoting maturation of cardiomyocytes, comprising contacting cardiomyocytes with an agent that modulates GLI signaling, wherein the modulation of GLI signaling induces maturation of the cardiomyocytes, wherein in the cardiomyocytes are human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) and wherein the agent is GANT61.
Claims18
A method for promoting maturation of cardiomyocytes, comprising contacting cardiomyocytes with an agent that modulates GLI signaling, wherein the modulation of GLI signaling induces maturation of the cardiomyocytes, wherein in the cardiomyocytes are human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs).
A method for inducing maturation of human induced pluripotent stem cell-derived cardiomyocytes, comprising:
- contacting the cardiomyocytes with GANT61 in culture, wherein the GANT61 inhibits GLI signaling and thereby promotes structural, electrophysiological, and metabolic maturation of the cardiomyocytes.
A cardiomyocyte maturation culture system, comprising
- cardiomyocytes derived from pluripotent stem cells;
- and
- a GLI signaling modulator present in culture medium in an amount sufficient to promote cardiomyocyte maturation at a concentration of 0.5 μM to 2.0 μM,
- wherein the GLI signaling modulator is capable of activating a PGC1α-mediated metabolic maturation pathway.
Dependencies are inferred from the claim text.