Apparatus for quantitative study of cellular interactions with mechanical waves
AssigneeArizona Board of Regents on Behalf of the University of Arizona
InventorsJian GU, Frederic ZENHAUSERN, Adam AKKAD, Nabhan FAKRUDIN
Life of this patent
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Abstract
Provided herein are specially-configured apparatus for maximizing mechanical or acoustic transmission so that a biological material supported by the apparatus reliably experiences a mechanical or acoustic wave that has minimally degraded. In this manner, a biomarker may be reliably monitored and the effect of the mechanical or acoustic wave on the biological material determined. The platform is used to manipulate a cellular interaction with the mechanical or acoustic wave. Related methods of using and making are provided.
Claims35
An apparatus for manipulating a cellular interaction with a mechanical wave comprising:
- a bottom layer forming a first fluidic channel;
- a top layer forming a second fluidic channel;
- an intermediate membrane positioned between the first fluidic channel and the second fluidic channel, wherein the intermediate membrane comprises a receiving surface, wherein the intermediate layer is in fluidic contact with the first fluidic channel and the second fluidic channel;
- wherein the first and/or second fluidic channels and/or the receiving surface of the intermediate membrane is configured to support a biological material;
- wherein the bottom layer, top layer and intermediate membrane are independently configured for mechanical wave transparency;
- a mechanical wave generator configured to provide the mechanical wave to the biological material;
- a biomarker parameter monitor configured to monitor a change in one or more biomarkers after provision of the mechanical wave.
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A method of making an apparatus for the study of cellular interaction with ultrasound, the method comprising the steps of:
- providing a bottom layer having a first fluidic channel;
- providing a top layer having a second fluidic channel;
- positioning an intermediate layer between the first fluidic channel and the second fluidic channel to form the first and second fluidic channels separated by the intermediate layer;
- providing a biological material to the first fluidic channel and/or the second fluidic channel and/or the receiving surface of the intermediate membrane;
- generating a mechanical waveform, wherein the top layer, bottom layer and intermediate membrane are configured to be mechanically transparent;
- mechanically stimulating the biological material with the mechanical waveform;
- and
- monitoring a change in one or more biomarkers after the mechanical stimulating step using a biomarker parameter monitor.
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Dependencies are inferred from the claim text.