Violet Excitable Tandem Dyes, and Methods for Making and Using the Same
OwnerGlenn Peter Bartholomew, David Michel Moureau +1inventors
InventorsGlenn Peter Bartholomew, David Michel Moureau, Itzel Patricia Vaca
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Abstract
Violet excitable tandem dyes are provided. Tandem dyes of embodiments of the invention include: a non-conjugated polymeric backbone; one or more violet excitable donor fluorophores linked to the non-conjugated polymeric backbone; and one or more acceptor fluorophores linked to the non-conjugated polymeric backbone; where the donor and acceptor fluorophores are in energy transfer relationship. Also provided are methods of making and using the tandem dyes, as well as kits that include the dyes and find use in embodiments of the methods.
Claims27
1-20. (canceled)
A method of evaluating a sample for presence of a target analyte, the method comprising:
- (a) contacting the sample with a labeled specific binding member that specifically binds the target analyte to produce a labeled sample, wherein the labeled specific binding member comprises a specific binding member conjugated to a tandem dye comprising:
- a non-conjugated polymeric backbone;
- one or more violet excitable donor fluorophores linked to the non-conjugated polymeric backbone;
- and
- one or more acceptor fluorophores linked to the non-conjugated polymeric backbone,
- wherein the donor and acceptor fluorophores are in energy transfer relationship;
- and
- (b) assaying the labeled composition for the presence of a labelled specific binding member-target analyte binding complex to evaluate whether the target analyte is present in the sample.
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40-43. (canceled)
45-62. (canceled)
A kit comprising:
- a tandem dye comprising:
- a non-conjugated polymeric backbone;
- one or more violet excitable donor fluorophores linked to the non-conjugated polymeric backbone;
- and
- one or more acceptor fluorophores linked to the non-conjugated polymeric backbone, wherein the donor and acceptor fluorophores are in energy transfer relationship.
66-82. (canceled)
Dependencies are inferred from the claim text.