Processes and systems for preparing cellular or viral membranes and nanoparticles
AssigneeArytha Biosciences, LLC
InventorsWeiwei GAO, Che-Ming Jack HU
Life of this patent
Estimating the term…
Abstract
The present invention relates to processes and systems for preparing nanoparticles, cellular or viral membranes and/or cellular or viral membrane coated nanoparticles using or comprising, inter alia, a multi-inlet vortexing reactor, tangential flow filtration (TFF) and/or a high shear fluid processor such as a microfluidizer (or a microfluidizer processor). The present invention also relates to the nanoparticles, cellular or viral membranes and/or cellular or viral membrane coated nanoparticles prepared by the present processes and systems, and the uses and/or applications of the nanoparticles, cellular or viral membranes and/or cellular or viral membrane coated nanoparticles.
Claims24
1-35. (canceled)
A system for preparing a nanoparticle, which system comprises:
- 1) a multi-inlet vortexing reactor that is configured to mix a material for forming a nanoparticle in an organic solvent and an aqueous phase to form a composition comprising said nanoparticle;
- and
- 2) a tangential flow filtration (TFF) system that is configured to reduce the amount of or to remove said organic solvent from said composition.
37-80. (canceled)
A system for preparing a cellular or viral membrane, which system comprises:
- 1) means for lysing a cell, a cellular vesicle or a virus to obtain a composition comprising a cellular or viral membrane and a non-membrane cellular or viral moiety;
- and
- 2) a tangential flow filtration (TFF) system that is configured to separate said cellular or viral membrane from said non-membrane cellular or viral moiety.
82-137. (canceled)
A system for preparing a cellular or viral membrane coated nanoparticle, which system comprises:
- 1) a system for preparing a nanoparticle core, which system comprises:
- a) a multi-inlet vortexing reactor that is configured to mix a material for forming a nanoparticle core in an organic solvent and an aqueous phase to form a composition comprising said nanoparticle core;
- and b) a tangential flow filtration (TFF) system that is configured to reduce the amount of or to remove said organic solvent from said composition;
- 2) a system for preparing a cellular or viral membrane, which system comprises:
- a) means for lysing a cell, a cellular vesicle or a virus to obtain a composition comprising a cellular or viral membrane and a non-membrane cellular or viral moiety;
- and b) a tangential flow filtration (TFF) system that is configured to separate said cellular or viral membrane from said non-membrane cellular or viral moiety;
- and
- 3) a high shear fluid processor that is configured to mix said nanoparticle inner core comprising said material with said cellular membrane derived from a cell or a membrane derived from a virus to form a nanoparticle comprising said inner core and an outer surface comprising said cellular membrane or viral membrane.
Dependencies are inferred from the claim text.