Slc4a1 (band 3) binding single domain antibodies for increased blood retention
OwnerJoseph BALTHASAR, Brandon BORDEAU +1inventors
InventorsJoseph BALTHASAR, Brandon BORDEAU, Toan Duc NGUYEN
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Abstract
Provided are binding partners that specifically bind to erythrocyte protein SLC4A1 transporter (Band 3) that is highly expressed on erythrocytes. The binding partners are provided as single-specific, bi-specific or multi-specific agents that have improved half-life and/or are effective at a lower concentration. A second component of a bispecific binding partner can bind to a payload-binding selectivity enhancer (PBSE), such as a drug that has been detached from an anti-body drug conjugate. In non-limiting embodiments the PBSE specifically binds to a drug that was administered to an individual as a component of an ADC.
Claims12
A single chain binding partner that specifically binds to SLC4A1 transporter/Band 3, wherein the single chain binding partner comprises or consists of a sequence selected from:
- >RB12
- (SEQ ID NO:
- 1)
- QVQLVQSGGGLVQAGGSLRLSCAASGSSSSIIAMGWYRQAPGKQRELVATISNGDTT
- NYIDSVKGRFTISRDNAKNTVYLQMASLEPEDTAVYYCNARQTKWYAAGYEYRTYDY
- WGQGTQVTVSS
- >RMC1
- (SEQ ID NO:
- 2)
- QVQLVQSGGGLVQAGGSLRLSCAASGSSSSIIAMGWYRQAPGKQRELVATISNGDTT
- NYIDSVKGRFTISRDNAKNTVYLQMASLEPEDTAVYYCNARQTKWYAAGYKYRTYDY
- WGQGTQVTVSS
- >RA8
- (SEQ ID NO:
- 3)
- QVQLVQSGGGLVQAGGSLRLSCAASERTFSTYAMGWFRQIPGKERLFVAAVNWNGK
- TIRYADSVKGRFTISRDNAKNTIALQMNSLKPEDTAVYYCALRSTPMYFTNLASQE
- SYNYWGPGTQVTVSS
- >RD1
- (SEQ ID NO:
- 4)
- QVQLVQSGGGLVQAGGSLRLSCAASGSSSSIIAMGWYRQAPGKQRELVATISNGDTT
- NYIDSVKGRFTISRDNAKNTVYLQMASLEPEDTAVYYCNARQTKWYAAGYEYRTYDY
- WGQGTQVTVSS
- >RD11
- (SEQ ID NO:
- 5)
- EVQLVESGGGLVQPGGSLRLSCAASGRIFSISNMGWYRQAPGKQRELVATITSGGSTN
- YGDSVKGRFTISMANAKNAVYLQMNSLKPEDTAVYYCNAGISRRTGTYSGGRYSDYWA
- QGTQVTVSS
- >RE8
- (SEQ ID NO:
- 6)
- QVQLVQSGGGLVQPGGSLRLSCAASGRVSEINTMGWYRQAPGKQRELVALITSTSST
- MYSDSVKGRFTVSRDVAKNMVYLQMNSLKPEDTAVYYCNARQTKWYAAGYEYRTYD
- YWGQGTQVTVSS
- >RG10
- (SEQ ID NO:
- 7)
- QVQLQESGGGLVQAGGSLRLSCAASERTFSTYAMGWFRQTPGKERLFVAAVNWNG
- KTIRYADSVKGRFTISRDNAKNTMSLQMNSLKPEDTAVYYCALRSTPMYFTNLASQESY
- NYWGPGTQVTVSS
- >RH5
- (SEQ ID NO:
- 8)
- QVQLVQSGGGLVQPGGSLRLSCAASGRIFSISNMGWYRQAPGKQRELVATITSGGSTN
- YGDSVKGRFTISMVNAKNAVYLQMNSLKPEDTAVYYCNAGISRRTGTYSGGRYSDYWG
- QGTQVTVSS
- >RMAI
- (SEQ ID NO:
- 9)
- QVQLVQSGGGLVQAGGSLRLSCAASGSSSSIIAMGWYRQAPGKQRELVATISNGDTT
- NYTDSVKGRFTISRDKAKNTVYLQMASLEPEDTAVYYCNARQTKWYAAGYVYRTYDY
- WGQGTQVTVSS
- >RMDI
- (SEQ ID NO:
- 10)
- QVQLVQSGGGLVQPGGSLRLGCAASGRVSEINTMGWYRQAPGKQRGLVALITSTSST
- MYSDSVKGRFTVSRDVAKNMVYLQMNSLKPEDTAVYYCNARLTRWYAAGYKYRTYD
- YWGQGTQVTVSS
- >RMF1
- (SEQ ID NO:
- 11)
- QVQLVQSGGGLVQAGGSLRLSCAASGSSSSIIAMGWYRQAPGKQRELVATISNGDTT
- NYIDSVNGRFTISRDNAKNTVYLQMTSLEPEDTAVYYCHARQTKWYAAGYKYRTYDY
- WGQGTQVTVSS
- >RMG1
- (SEQ ID NO:
- 12)
- QVQLVQSGGGLVQPGGSLRLSCAASGRVSEINTMGWYRLAPGKQRELVALITSTSST
- MYSDSVKGRFTVSRDVAKNMVYLQMNSLKPEDTAVYYCNARQTKWYAAGYKYRTYD
- YWGQGTQVTVSS
- >RMC2
- (SEQ ID NO:
- 13)
- QVQLVQSGGGLVQPGGSLRLSCAASGRVSEINTMGWYRQAPGKQRELVALITSTSST
- MYSDSVKGRFTVSRDVAKNMVYLQMNSLKPEDTAVYYCNARQTIWYAAGYKYRTYDY
- WGLGTQVTVSS
- >RME2
- (SEQ ID NO:
- 14)
- QVQLVQSGGGLVQSGGSLRLSCAASGSSSSIIAMGWYRQAPGKQRELVATISNGDTT
- NYIDSVKGRFTISRDNAKNTVYLQMASLEPEDTAVYYCNARQTKWYAAGYEYRTYDY
- WGQGTQVTVSS
- >RMF2
- (SEQ ID NO:
- 15)
- QVQLVQSGGGLVQAGGSLRLSCAASGSSSSIIAMGWYRQAPGKQRELVATISNNDTT
- NYIDSVKGRFTISRDNAKNTVYLQMASLEPEDTAVYYCNARQTKWYAAGYKYRTYDY
- WGQGTQVTVSS
- >RMG2
- (SEQ ID NO:
- 16)
- QVQLVQSGGGQVQAGGSLRLSCAASGSSSSIIAMGWYRQAPGKQRELVATISNGDTT
- NYIDSVKGRFTISRDNAKNTVYLQMASLEPEDTAVYYCNARQTKWYAAGYKYRTYDY
- WGQGTQVTVSS
- >RMH2
- (SEQ ID NO:
- 17)
- QVQLVQSGGGLAQAGGSLRLSCAASGSSSSIIAMGWYRQAPGKQRELVATISNGDTT
- NYIDSVKGRFTISRDNAKNTVYLQMASLEPEDTAVYYCNARQTKWYAAGYKYRTYDY
- WGQGTQVTVSS
- >RMB3
- (SEQ ID NO:
- 18)
- QVQLVQSGGGLVQPGGSLRLSCAASGRVSEINTMGWFRQAPGKQRELVALITSTSST
- MYSDSVKGRFTVSRDVAKNMVYLQMNSLKPEDTAVYYCNARQTKWYAAGYKYRTYD
- YWGQGTQVTVSS
- >RMD3
- (SEQ ID NO:
- 19)
- QVQLVQSGGGLVQAGGSLRLSCAASGSSSSIIAMGWYRQAPGKQRELVATISNGDTT
- NYIDSVKGRFTISRDNAKNTVYLQMASLEPEDTAVYYCNARQTRWYAAGYKYRTYDY
- WGQGTQVTVSS
- >RME3
- (SEQ ID NO:
- 20)
- QVQLVQSGGGLVQPGGSLRLSCAASGRVSEINTVGWYRQAPGKORELVALFTSTSST
- MYSDSVKGRFTVSRDVAKNMVYLQMNSLRPEDTAVYYCNARQTKWYAAGYKYRTYD
- YWGQGTQVTVSS
- and
- >RMG3
- (SEQ ID NO:
- 21)
- QVQLVQSGGGLVQAGGSLRLSCAASGSSSSIIAMGWYRQAPGKQRELVATISNGDTT
- NYIDSVKGRFTISRDNAKNTVYLRMASLEPEDTAVYYCNARQTKWYAAGYKYRTYDY
- WGQGTQVTVSS
Dependencies are inferred from the claim text.