Methods and compositions for displaying peptide libraries

Abstract

A non-naturally occurring test molecule for use in a cell-based screening method, wherein the molecule comprises: a molecule of interest comprising a message/address peptide; a linker sequence; and an anchor sequence which anchors the test molecule to a plasma membrane of the cell. A method of screening to determine interaction between a capture protein and a molecule of interest, the method comprising the steps of: providing the test molecule; providing a eukaryotic cell, wherein said eukaryotic cell comprises a non-endogenous capture protein; allowing the molecule of interest and the non-endogenous capture protein to interact; and determining interaction between the molecule of interest and the capture protein.

Dependencies are inferred from the claim text.