Method for isolating fetuin-a
AssigneePreviPharma GmbH
InventorsStephan T. KIESSIG, Rebecca Michaela REINIG, Tina BERGER, Satish Kumar DEVARAPU, Wolfgang TESCHNER
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Abstract
The present invention relates to a method for isolating Fetuin-A, comprising a step of contacting the fluid containing Fetuin-A to a solid phase that bears moieties of an anthraquinone compound, in particular Reactive Blue 2. Furthermore, the present invention refers a composition comprising Fetuin-A obtainable from such method and uses thereof. Moreover, the invention refers to a method for determining the activity of Fetuin-A.
Claims20
1-15. (canceled)
A method for isolating Fetuin-A from a fluid containing Fetuin-A, wherein the method comprises the following steps:
- (i) contacting the fluid containing Fetuin-A with a solid phase comprising a solid material and moieties of an anthraquinone compound;
- (ii) allowing adhesion of the Fetuin-A to the solid phase;
- (iii) at least partly removing the fluid from the solid phase and optionally washing the solid phase with a washing buffer;
- and
- (iv) contacting the solid phase with an elution buffer and thereby eluting the Fetuin-A from the solid phase.
A method for determining the activity of Fetuin-A, the method comprising the following steps:
- (i) providing a solution containing:
- (a) a first solution containing soluble calcium salt,
- (b) a second solution containing a soluble phosphate salt, and
- (c) a sample containing the Fetuin-A;
- (ii) mixing the components provided in step (i) with each other;
- and
- (iii) determining the precipitation time of insoluble calcium phosphate,
- wherein an elongated precipitation time indicates activity of Fetuin-A.
Dependencies are inferred from the claim text.