Double networked 3d-printed biomaterials
AssigneeThe Brigham and Women's Hospital, Inc.
InventorsYu Shrike Zhang, Xiao Kuang
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Abstract
A 3D-printed biomaterial is described that comprises a double-networked hydrogel comprising gelatin or gelatin methacryloyl and alginate, and cells. Methods of making the 3D-printed biomaterial by extruding a hydrogel comprising gelatin or gelatin methacryloyl and alginate from a 3D-printer and converting the hydrogel to a double-networked hydrogel by cross-linking the gelatin with a first cross-linking method and cross-linking the alginate with a second cross-linking method are also described. Methods of treating a damaged or diseased blood vessel and testing vasoactive drugs using 3D-printed blood vessels are also described.
Claims44
A 3D-printed biomaterial, comprising a double-networked hydrogel comprising gelatin or gelatin methacryloyl (GelMA), alginate, and cells.
A method of making a 3D-printed biomaterial, comprising
- extruding a hydrogel comprising gelatin or GelMA and alginate from a 3D-printer;
- converting the hydrogel to a double-networked hydrogel by
- a) cross-linking the alginate with a first cross-linking method;
- and
- b) cross-linking the gelatin or GelMA with a second cross-linking method.
A method of treating a damaged or diseased blood vessel in a subject, comprising attaching a 3D printed blood vessel to the damaged or diseased blood vessel of the subject and rerouting blood flow so that blood flows through the 3D printed blood vessel, wherein the 3D-printed blood vessel comprises a hollow tube comprising a middle layer comprising a double-networked hydrogel comprising gelatin and alginate, an inner layer comprising endothelial cells, and an outer layer comprising smooth muscle cells.
A method of testing a vasoactive drug, comprising contacting a 3D-printed blood vessel with an effective amount of a vasoactive drug, and observing the effect of the vasoactive drug on the 3D-printed blood vessel, wherein the 3D-printed blood vessel comprises a hollow tube comprising a middle layer comprising a double-networked hydrogel comprising gelatin and alginate, an inner layer comprising endothelial cells, and an outer layer comprising smooth muscle cells.
Dependencies are inferred from the claim text.