Methods and compositions for dental care

A method of making a tri-layered collagen composition comprising

• a) preparing a collagen slurry;
• b) adding bioactive glass to the collagen slurry, forming a bioactive glass collagen slurry mix;
• c) coating the first side of a collagen base, having a first side and a second side, with the bioactive glass collagen slurry mix of step b), forming a single-coated collagen base;
• d) drying the single-coated collagen base;
• e) coating the second side of the collagen base with a bioactive glass collagen slurry mix, forming a double-coated collagen base;
• f) drying the double-coated collagen base, thereby forming a dried collagen product;
• g) pressing the dried collagen product, thereby forming a pressed, dried collagen product;
• h) drying the pressed, lyophilized collagen product;
• thereby forming a dried, pressed collagen product;
• i) crosslinking the dried collagen product of h) thereby forming a crosslinked collagen product;
• j) drying the crosslinked collagen product of i), thereby forming a dried, crosslinked collagen product;
• k) pressing the dried, crosslinked collagen product of j), thereby forming a pressed, dried, crosslinked collagen product;
• and
• l) drying the pressed, dried, crosslinked collagen product of k), thereby forming a tri-layered collagen product.

A method of making a bilayer collagen composition comprising

• a) preparing a collagen slurry;
• b) adding bioactive glass to the collagen slurry, forming a bioactive glass collagen slurry mix;
• c) coating one side of a collagen base with the bioactive glass collagen slurry mix, forming a single-coated collagen base;
• d) drying the single-coated collagen base, thereby forming a dried collagen product;
• e) pressing the dried collagen product, thereby forming a pressed, dried collagen product;
• f) drying the pressed, dried collagen product, thereby forming a dried collagen product;
• g) crosslinking the dried collagen product, thereby forming a crosslinked collagen product;
• h) drying the crosslinked collagen product, thereby forming a dried, crosslinked collagen product;
• i) pressing the dried, crosslinked collagen product, thereby forming a pressed, dried, crosslinked collagen product
• j) drying the pressed, dried, crosslinked collagen product, thereby forming a bilayered collagen product.

A method of making a tri-layered collagen composition comprising

• a) preparing a collagen slurry;
• b) drying a least a portion of the collagen slurry;
• c) pressing the lyophilized collagen slurry to form a collagen base;
• d) perforating the first side of a collagen base, having a first side and a second side, thereby forming a perforated collagen base;
• e) adding bioactive glass to the first side of the perforated collagen base;
• f) coating the first side of the perforated collagen base comprising bioactive glass with the collagen slurry, thereby forming a single-coated collagen base;
• g) drying the single-coated collagen base;
• h) perforating the second side of the collagen base;
• i) adding bioactive glass to the second side of the perforated collagen base;
• j) coating the second side of the perforated collagen base comprising bioactive glass with the collagen slurry, thereby forming a double-coated collagen base;
• k) lyophilizing the double-coated collagen base, thereby forming a lyophilized collagen product.
• l) pressing the lyophilized collagen product, thereby forming a pressed, lyophilized collagen product;
• m) drying the pressed, lyophilized collagen product;
• forming a dried collagen product;
• n) crosslinking the dried collagen product of k), thereby forming a crosslinked collagen product;
• o) drying the crosslinked collagen product of l), thereby forming a dried, crosslinked collagen product;
• p) pressing the dried, crosslinked collagen product of m), thereby forming a pressed, dried, crosslinked collagen product
• q) drying the pressed, dried, crosslinked collagen product of n), thereby forming a tri-layered collagen product.

A method of making a bilayer collagen composition comprising

• a) preparing a collagen slurry;
• b) drying a least a portion of the collagen slurry;
• c) pressing the lyophilized collagen slurry to form a collagen base;
• d) perforating one side of a collagen base;
• e) adding bioactive glass to the perforated side of the collagen base;
• f) coating the perforated collagen base side comprising bioactive glass with the collagen slurry, thereby forming a single-coated collagen base;
• g) drying the single-coated collagen base;
• h) lyophilizing the single-coated collagen base, thereby forming a lyophilized collagen product.
• i) pressing the lyophilized collagen product of f), thereby forming a pressed, lyophilized collagen product;
• j) drying the pressed, lyophilized collagen product of g);
• forming a dried collagen product
• k) crosslinking the dried collagen product of h), thereby forming a crosslinked collagen product;
• l) drying the crosslinked collagen product of i), thereby forming a dried, crosslinked collagen product;
• m) pressing the dried, crosslinked collagen product of j), thereby forming a pressed, dried, crosslinked collagen product;
• n) drying the pressed, dried, crosslinked collagen product of k), thereby forming a tri-layered collagen product.

A method of making a trilayer collagen composition comprising a) preparing a collagen slurry;

• b) lyophilizing at least a portion of the collagen slurry;
• c) pressing the lyophilized collagen slurry, thereby forming a collagen base;
• d) adding bioactive glass to a collagen slurry, thereby forming a bioactive glass collagen slurry mix;
• e) coating the first side of the collagen base of c), having a first side and a second side, with the bioactive glass collagen slurry mix of d), thereby forming a single-coated collagen base;
• f) drying the single-coated collagen base;
• g) coating the second side of the single-coated collagen base with the bioactive glass collagen slurry mix, thereby forming a double-coated collagen base;
• h) lyophilizing the double-coated collagen base of g), thereby forming a lyophilized collagen product,
• i) pressing the lyophilized collagen product of h), thereby forming a pressed, lyophilized collagen product;
• j) drying the pressed, lyophilized collagen product of i);
• thereby forming a dried collagen product;
• k) crosslinking the dried collagen product of j), thereby forming a crosslinked collagen product;
• l) drying the crosslinked collagen product of k), thereby forming a dried, crosslinked collagen product;
• m) pressing the dried, crosslinked collagen product of l), thereby forming a pressed, dried, crosslinked collagen product;
• and
• n) drying the pressed, dried, crosslinked collagen product of m), thereby forming a tri-layered collagen product

A method of making a bilayer collagen composition comprising

• a) preparing a collagen slurry;
• b) lyophilize at least a portion of the collagen slurry;
• c) pressing the lyophilized collagen slurry to form a collagen base;
• d) adding bioactive glass to a collagen slurry, forming a bioactive glass collagen slurry mix;
• e) coating one side of the collagen base with the bioactive glass collagen slurry mix, thereby forming a single-coated collagen base;
• f) drying the single-coated collagen base;
• g) lyophilizing the single-coated collagen base, thereby forming a lyophilized collagen product.
• h) pressing the lyophilized collagen product of g), thereby forming a pressed, lyophilized collagen product;
• i) drying the pressed, lyophilized collagen product of h);
• thereby forming a dried collagen product;
• j) crosslinking the dried collagen product of i), thereby forming a crosslinked collagen product;
• k) drying the crosslinked collagen product of j), thereby forming a dried, crosslinked collagen product;
• l) pressing the dried, crosslinked collagen product of k), thereby forming a pressed, dried, crosslinked collagen product;
• and
• m) drying the pressed, dried, crosslinked collagen product of l), thereby forming a bilayered collagen product.

A method of making a (above) composition comprising

• a) preparing a collagen slurry;
• b) lyophilizing at least a portion of the collagen slurry;
• c) pressing the lyophilized collagen slurry to form a collagen base;
• d) perforating the first side of a collagen base, having a first side and a second side, thereby forming a perforated collagen base;
• e) adding bioactive glass to the first side of the perforated collagen base;
• f) coating the first side of the perforated collagen base comprising bioactive glass with the collagen slurry, thereby forming a single-coated collagen base;
• g) drying the single-coated collagen base;
• h) perforating the second side of the collagen base;
• i) adding bioactive glass to the second side of the perforated collagen base;
• j) coating the second side of the perforated collagen base comprising bioactive glass with the collagen slurry, thereby forming a double-coated collagen base;
• k) lyophilizing the double-coated collagen base, thereby forming a lyophilized collagen product.
• l) pressing the lyophilized collagen product of k), thereby forming a pressed, lyophilized collagen product;
• m) drying the pressed, lyophilized collagen product of l);
• thereby forming a dried collagen product
• n) crosslinking the dried collagen product of m),
• o) drying the crosslinked collagen product of n), thereby forming a dried, crosslinked collagen product;
• p) pressing the dried, crosslinked collagen product of o), thereby forming a pressed, dried, crosslinked collagen product;
• q) drying the pressed, dried, crosslinked collagen product of p), thereby forming a tri-layered collagen product.

A method of making a bilayer collagen composition comprising

• a) preparing a collagen slurry;
• b) lyophilizing at least a portion of the collagen slurry;
• c) pressing the lyophilized collagen slurry to form a collagen base;
• d) perforating one side of a collagen base;
• e) adding bioactive glass to the perforated collagen base;
• f) coating the side of the perforated collagen base comprising bioactive glass with the collagen slurry, forming a single-coated collagen base;
• g) drying the single-coated collagen base;
• h) lyophilizing the single-coated collagen base, thereby forming a lyophilized collagen product.
• i) pressing the lyophilized collagen product of h),thereby forming a pressed, lyophilized collagen product;
• j) drying the pressed, lyophilized collagen product of i);
• thereby forming a dried collagen product
• k) crosslinking the dried collagen product of j), thereby forming a crosslinked collagen product,
• l) drying the crosslinked collagen product of k), thereby forming a dried, crosslinked collagen product;
• m) pressing the dried, crosslinked collagen product of l), thereby forming a pressed, dried, crosslinked collagen product;
• n) drying the pressed, dried, crosslinked collagen product of m), thereby forming a bilayered collagen product.

A method of making a monolayer collagen composition comprising

• a) preparing a collagen slurry;
• b) adding bioactive glass to the collagen slurry, forming a bioactive glass collagen slurry mix;
• and
• c) optionally, drying the bioactive glass collagen slurry mix, thereby forming a dried collagen product.