Vaccine Compositions
AssigneeSeqirus Inc.
InventorsDouglas Widman, Palaniappan Ramanathan
Life of this patent
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Abstract
The present disclosure relates to RNAs and other polynucleotides encoding an antigen, which can be used in vaccine compositions. In some examples, the RNA also encodes a chemoattractant, such as chemerin, C-X-C motif chemokine ligand 9 (CX-CL9), C-X-C motif chemokine ligand 10 (CXCL10) or C-X-C motif chemokine ligand 11 (CXCL11). The present disclosure also relates to use of the RNAs and compositions in methods of inducing an immune response in a subject.
Claims76
A self-replicating RNA comprising a first nucleotide sequence encoding an antigen operably linked to a regulatory element, and a second nucleotide sequence encoding a chemoattractant operably linked to a regulatory element.
An immunogenic composition comprising:
- a) a self-replicating RNA comprising a first nucleotide sequence encoding an antigen operably linked to a regulatory element;
- and
- b) a chemoattractant polypeptide, optionally selected from the group consisting of chemerin, CXCL9, CXCL10 and CXCL11.
A polynucleotide comprising:
- a) a first nucleotide sequence encoding an antigen operably linked to a regulatory element;
- and
- b) a second nucleotide sequence encoding a chemoattractant operably linked to a regulatory element selected from the group consisting of a SG promoter and an internal ribosome entry site (IRES),
- optionally wherein the chemoattractant is selected from the group consisting of chemerin, C-X-C motif chemokine ligand 9 (CXCL9), C-X-C motif chemokine ligand 10 (CXCL10) and C-X-C motif chemokine ligand 11 (CXCL11).
A conventional mRNA (cRNA) comprising:
- a) a first nucleotide sequence encoding an antigen operably linked to a regulatory element;
- and
- b) a second nucleotide sequence encoding a chemoattractant operably linked to a regulatory element selected from the group consisting of a SG promoter and an internal ribosome entry site (IRES),
- optionally wherein the chemoattractant is selected from the group consisting of chemerin, C-X-C motif chemokine ligand 9 (CXCL9), C-X-C motif chemokine ligand 10 (CXCL10) and C-X-C motif chemokine ligand 11 (CXCL11).
Dependencies are inferred from the claim text.