Composition for kidney treatment using omentum, a medical kit for kidney treatment, including the same, and film for kidney treatment, including cured product thereof

A method for manufacturing of film for kidney treatment using omentum, comprising:

• chopping an extracted omentum or an omentum tissue to obtain a chopped omentum tissue having a diameter of about 5 mm or less;
• forming a mixture of the chopped omentum tissue with normal saline;
• grinding and filtering the mixture through a first filter having a pore diameter of 1 to 3 mm to obtain a first filtrate;
• grinding and filtering the first filtrate with a second filter having a pore diameter of 400 to 800 μm to obtain a second filtrate;
• grinding and filtering the second filtrate with a third filter having a pore diameter of 5 to 200 μm to obtain a third filtrate;
• obtaining a micronized omentum-derived tissue extract from the third filtrate;
• preparing a first liquid containing the micronized omentum-derived tissue extract and fibrinogen;
• preparing a second liquid containing thrombin;
• applying the first liquid to form a first layer;
• and
• applying the second liquid onto the first layer to form a film for kidney treatment by crosslinking the fibrinogen and the thrombin;
• wherein the micronized omentum-derived tissue extract comprises omentum-derived tissue particles having an average particle diameter of 5 μm to 200 μm,
• wherein the micronized omentum-derived tissue extract comprises omentum-derived stromal cells, and an omentum-derived extracellular matrix, and
• wherein the content of the micronized omentum-derived tissue extract in the first liquid is 40 vol% to 90 vol%.