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US 2026/0306405 A1Published application
Leveraging lipid-protein interactions to engineer spatial organization in cell-free systems
OwnerJustin Alexander Peruzzi, Neha Prashant Kamat +1inventors
InventorsJustin Alexander Peruzzi, Neha Prashant Kamat, Jan Steinkuehler
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Abstract
The present disclosure provides methods and compositions for controlling the localization of proteins in lipid structures. The disclosure also provides methods for selecting appropriate proteins for desired localization and distribution in lipid structures based on hydrophobic thickness.
Claims8
-13. (canceled)
A lipid structure prepared by a method comprising:
- providing a first protein, the first protein having a first hydrophobic thickness;
- providing a lipid structure comprising a lipid bilayer, the lipid bilayer comprising a first domain having a second hydrophobic thickness;
- and
- combining the first protein and the lipid structure under conditions that promote integration of the first protein into the first domain of the lipid bilayer,
- wherein the first protein traverses the lipid bilayer, and
- wherein the first hydrophobic thickness and the second hydrophobic thickness have a difference of no greater than about 5 angstroms.
3 more elements
-27. (canceled)
A lipid structure prepared by a method comprising:
- providing two or more proteins, each protein having a protein hydrophobic thickness, wherein each protein hydrophobic thickness has a difference of greater than about 5 angstroms;
- providing a lipid structure comprising a lipid bilayer having two or more domains, wherein each of the two or more domains has a bilayer hydrophobic thickness, and wherein each bilayer hydrophobic thickness has a difference of greater than about 5 angstroms;
- and
- combining the proteins and the lipid structure under conditions that promote integration of the proteins into the lipid bilayer
- wherein the proteins traverse the lipid bilayer, and
- wherein each protein thickness is no greater than 5 angstroms different than the bilayer hydrophobic thickness of one of the two or more domains.
3 more elements
A composition for preparing a synthetic vesicle having a transmembrane protein comprising:
- the synthetic vesicle;
- and
- a cell free protein expression system for expressing the transmembrane protein,
- wherein the transmembrane protein has a protein hydrophobic thickness;
- wherein the synthetic vesicle comprises a lipid bilayer having a domain, wherein the domain has a bilayer hydrophobic thickness;
- wherein the protein thickness is no greater than 5 angstroms different than the bilayer hydrophobic thickness.
3 more elements
-42. (canceled)
Dependencies are inferred from the claim text.