System and method for hyperspectral imaging and mapping of tissue oxygen saturation

An imaging system comprising:

• a white-light source configured to output white light to illuminate tissue;
• a first-wavelength light source producing light at a first wavelength primarily absorbed by deoxyhemoglobin;
• a second-wavelength light source producing light at a second wavelength primarily absorbed by oxyhemoglobin;
• a scope configured to receive the white light, the first-wavelength light, and the second-wavelength light reflected from the tissue;
• at least one camera coupled to the scope, the at least one camera configured to capture a plurality of frames of the tissue illuminated by at least one of the white light, the first-wavelength light, and the second-wavelength light;
• an image processing unit configured to:
• activate the white-light source to produce white light to illuminate tissue;
• capture frames of a video feed while the tissue is illuminated with the white light;
• temporarily deactivate the white-light source at a first time;
• while the white-light source is deactivated at the first time:
• activate the first-wavelength light source to output the first-wavelength light;
• and
• receive a first single wavelength image of the tissue illuminated by the first-wavelength light;
• temporarily deactivate the white-light source at the second time;
• while the white light source is deactivated at a second time:
• activate the second-wavelength light source to output the second-wavelength light;
• and
• receive a second single wavelength image of the tissue illuminated by the second-wavelength light;
• and
• generate a hyperspectral image based on the first single wavelength image and the second single wavelength image.

A method for imaging perfusion of tissue, the method comprising:

• activating a white-light source to illuminate tissue;
• receiving through a scope the white light reflected from the tissue;
• capturing, at a camera coupled to the scope, a plurality of frames of the tissue for a video feed;
• temporarily deactivating the white-light source at a first time;
• while the white-light source is deactivated at the first time:
• activating a first-wavelength light source to illuminate the tissue with a first-wavelength light;
• and
• capturing, with the camera, a first-wavelength frame of the tissue while the tissue is illuminated with the first-wavelength light;
• temporarily deactivating the white-light source at a second time;
• while the white-light source is deactivated at the second time:
• activating a second-wavelength light source to illuminate the tissue with a second-wavelength light;
• and
• capturing, with the camera, a second-wavelength frame of the tissue while the tissue is illuminated with the second-wavelength light;
• and
• generating, at an image processing unit, a hyperspectral image based on the first-wavelength frame and the second-wavelength frame.