Novel lactobacillus strains
AssigneeDsm IP Assets B.V.
InventorsPaul KLAASSEN, Jeroen SCHOEVERS, Thomas Hendrik ECKHARDT, Daniel Avraham SCHWARTZ
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Abstract
The present invention relates to novel Lactobacillus strains with extended shelf-life properties, i.e., capability to extend the shelf-life at ambient temperature of a food product, such as e.g., a fermented milk product, when added to such products, without heavily diminishing the antimicrobial, antifungal and/or anti-yeast effect of said strains. The invention furthermore relates to a method of generating such novel strains, particularly generation via adaptive evolution.
Claims14
A method for obtaining a mutant lactic acid bacterial strain, optionally a Lactobacillus bacterial strain, optionally capable of reducing post-acidification in a fermented milk product in comparison with a parent Lactobacillus bacterial strain, wherein the method comprises subjecting the parent lactic acid bacterial strain to an adaptive evolution, said adaptive evolution comprising a series of evolutionary changes, wherein each next descendent strain of a preceding strain is subjected to a higher environmental temperature than the preceding strain and wherein the mutant lactic acid bacterial strain when present in or added to a fermented milk product is increasing the pH in said fermented milk product in the range of 0.1 to 0.2 during storage of the fermented milk product at about 20° C. as compared to the pH measured under the same conditions in a fermented milk product comprising the parent Lactobacillus bacterial strain, and wherein the pH is determined after storage of said fermented milk product fermented with a starter culture over at least about 14 days at 20° C., and wherein said parent or mutant Lactobacillus bacterial strain is present in a concentration of at least about 107 cell forming units per gram (cfu/g).
Dependencies are inferred from the claim text.