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US 2026/0305618 A1Published application
Methods and compositions for microbial delivery of double stranded rna
AssigneeRnaissance AG LLC
InventorsJohn L. Killmer, Anil Kumar
Life of this patent
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Abstract
Methods and materials for improved delivery of dsRNA are presented. In particular, methods for controlling an insect pest by stably delivering large quantities of dsRNA to the insect pest are provided. Compositions comprising a bacterium containing a large quantity of dsRNA targeting an insect gene in which the dsRNA exhibits increased stability and activity are also provided.
Claims20
A microorganism genetically modified to control an insect pest, the microorganism comprising:
- (i) a promoter operably linked to a DNA sequence comprising a coat protein gene encoding a capsid protein or N-terminus of the capsid protein coding sequence (CDS);
- and
- (ii) a promoter operably linked to a DNA sequence encoding a dsRNA molecule that specifically inhibits expression of a target gene in the insect pest, wherein the dsRNA is at least 60% undegraded dsRNA after exposure of least 7 days to an aqueous environment relative to degradation of the dsRNA when isolated.
A system for delivering dsRNA to an insect pest, comprising:
- (a) water;
- and
- (b) in the water, a genetically modified microorganism comprising:
- (i) a promoter operably linked to a DNA sequence comprising a coat protein gene encoding a capsid protein or N-terminus of the capsid protein coding sequence (CDS);
- and
- (ii) a promoter operably linked to a DNA sequence encoding a dsRNA molecule that specifically inhibits expression of a target gene in an insect pest, wherein the dsRNA is at least 60% undegraded after at least 7 days in the water relative to the dsRNA when isolated.
3 more elements
Dependencies are inferred from the claim text.