Methods and systems for designing longevity-associated nucleic acid sequences using dnarp

A system for selecting a candidate nucleic acid sequence for age-reversal-associated use, the system comprising:

• one or more processors;
• and memory storing instructions that, when executed by the one or more processors, cause the system to:
• receive a target associated with a longevity-associated gene or a candidate nucleic acid sequence corresponding to the target;
• compute, for the candidate nucleic acid sequence, a sequence stability score according to a scoring convention that computes a weighted sum of (i) a CG occurrence count and (ii) an AT occurrence count under a stored counting convention;
• determine whether the sequence stability score satisfies a stability threshold;
• for a candidate nucleic acid sequence that satisfies the stability threshold, evaluate structural compatibility of the candidate nucleic acid sequence according to a helical parameter set comprising a pitch parameter and a groove ratio parameter;
• for a candidate nucleic acid sequence that satisfies the structural compatibility evaluation, select a coherence level for the candidate nucleic acid sequence from an allowed set of coherence levels;
• compute, using the sequence stability score and the selected coherence level, a predicted enhancement score for the candidate nucleic acid sequence;
• rank the candidate nucleic acid sequence relative to one or more other candidate nucleic acid sequences based on at least the predicted enhancement score;
• and select the candidate nucleic acid sequence for synthesis, construct assembly, editing, delivery, or validation based on the ranking.