Information acquisition method of acquiring information relating to nutritional state of cell group

An information acquisition method of acquiring information relating to a nutritional state of a cell group from an image including the cell group, the information acquisition method comprising:

• an image acquisition step of acquiring an image including at least one of a scattered light image of the cell group and an autofluorescence image of the cell group;
• an extraction step of extracting individual cell regions of the cell group in the image;
• an intensity distribution acquisition step of calculating intensity information of the individual cell regions, and further acquiring information relating to an intensity distribution of the cell group from the intensity information of the individual cell regions as a feature value of the cell group;
• and
• a nutritional state acquisition step of acquiring information relating to the nutritional state of the cell group, based on the information relating to the intensity distribution of the cell group, wherein
• the information relating to the nutritional state includes information relating to an amount of at least one selected from the group consisting of sugars, lactic acid, pyruvic acid, glutamine, glutamic acid, and lipids provided to the cell group.