Microscope and Microscopy Method

A Microscope comprising:

• an illumination beam path with a radiation source for emitting excitation light, the illumination beam path being configured to guide the excitation light into a sample region,
• a detection beam path with a microscope objective and with a detector for detecting emission light emitted by a sample in the sample region as a consequence of being irradiated by the excitation light, the detection beam path being configured to guide the emission light to the detector, and
• a control unit for evaluating the emission light detected by the detector,
• the control unit having a memory device in which original excitation spectra and emission spectra of dyes and, for different system configurations of the illumination beam path and of the detection beam path,
• spectral properties of the illumination beam path and
• spectral properties of the detection beam path are stored,
• wherein the control unit is configured to make use of
• the original excitation spectra and emission spectra of those dyes with which the sample is prepared,
• the spectral properties of the illumination beam path and the spectral properties of the detection beam path to ascertain values proportional to concentrations of the respective dyes at a location in the sample from measurement data measured by the detector for the relevant location in the sample for different system configurations.