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US 2026/0298929 A1Published application
Detection of molecular associations
AssigneeCalifornia Institute of Technology
InventorsMitchell Guttman, Mario R. Blanco, Ward Walkup, IV
Life of this patent
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Abstract
In some embodiments, methods of detecting an association between a query protein and a target moiety are described. In some embodiments, compositions are described. In some embodiments, kits are described.
Claims21
1-2. (canceled)
A method of detecting an association between a query protein and a target moiety, the method comprising:
- providing the query protein comprising a tag;
- contacting the query protein with a composition comprising the target moiety, whereby the target moiety associates with the query protein;
- applying a crosslinking agent or force to the query protein and the composition, thereby crosslinking the query protein to the target moiety associated therewith;
- covalently binding the tag to a substrate, thereby covalently immobilizing the query protein and crosslinked target moiety on the substrate;
- washing the immobilized query protein and crosslinked target moiety under denaturing conditions, wherein the query protein remains immobilized on the substrate;
- and
- detecting the target moiety associated with the query protein after said washing.
4 more elements
A composition comprising
- a query protein comprising a tag;
- a substrate, wherein the tag is covalently bound to the substrate;
- a target moiety crosslinked to the query protein;
- wherein the composition is under denaturing conditions.
1 more element
A method of detecting an association between a query protein and a target RNA, the method comprising:
- providing the query protein comprising a tag;
- wherein
- contacting the query protein with a composition comprising the target RNA, whereby the target RNA associates with the query protein;
- applying a crosslinking agent or force to the query protein and the composition, thereby crosslinking the query protein to the target RNA associated therewith;
- covalently binding the tag to a substrate, thereby covalently immobilizing the query protein and crosslinked target RNA on the substrate;
- washing the immobilized query protein and crosslinked target RNA under denaturing conditions, wherein the query protein remains immobilized on the substrate;
- and
- detecting the target RNA associated with the query protein after said washing.
5 more elements
Dependencies are inferred from the claim text.