Biosensors and methods for the determination of the monkeypox and chickenpox viruses belonging to the genus orthopoxvirus
AssigneeMugla Sitki Kocman Universitesi Strateji Gelistirme Daire Baskanligi
InventorsUlku ANIK, Yudum TEPELI BUYUKSUNETCI
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Abstract
A biosensor is provided, which has been developed for the diseases caused by monkeypox and chickenpox viruses with reference to their infection mechanisms and may be used for the diagnosis thereof, and a method for the determination of possible monkeypox and chickenpox viruses in individuals using the operating system of these biosensors is also provided.
Claims20
A method for determining a presence of monkeypox virus and chickenpox virus in a sample, comprising qualitatively or quantitatively measuring a presence of possible monkeypox virus and possible chickenpox virus in the sample by electrochemical methods using a biosensor comprising at least one glycosaminoglycan (GAG), wherein the at least one GAG selectively interacts with the monkeypox virus and the chickenpox virus as a bioreceptor, and the method comprises the following process steps:
- contacting the sample comprising the possible monkeypox virus or the possible chickenpox virus with the bioreceptor immobilized to an available working electrode,
- obtaining a biological interaction between the possible monkeypox virus or the possible chickenpox virus in the sample and the bioreceptor, and
- differentiating an impedance or a current on an electrode surface by the biological interaction obtained and ensuring an individual to understand whether the individual is sick or not by measuring a difference.
A method for determining a presence of a monkeypox virus or a chickenpox virus, comprising qualitatively or quantitatively measuring a presence of a possible monkeypox virus or a possible chickenpox virus in a sample by colorimetric methods using a biosensor comprising at least one GAG, wherein the at least one GAG selectively interacts with the monkeypox virus and the chickenpox virus as a bioreceptor, and the method comprises the following process steps:
- firstly adding a color-changing reactive compound to a reaction compartment,
- adding an appropriate bioreceptor into the reaction compartment,
- adding the sample obtained from a patient into the reaction compartment and stirring, and
- detecting a presence or an absence of a color change in the reaction compartment.
Dependencies are inferred from the claim text.